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KMID : 0378020070500090024
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New Medical Journal
2007 Volume.50 No. 9 p.24 ~ p.36
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Changes of Nociceptin Receptor by Fetal Hypoxia in Rat Brain
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Yoon Jun-Rho
Chung Eun-Yong
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Abstract
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Background: It has been suggested that nociceptin, a member of opioid peptides, influence the neural development. We examined the specific binding capacity of 125I-Tyr-nociceptin in rat of postnatal day 2 to 56, and their alteration by fetal hypoxic treatment.
Methods: Pregnant 230-250 g Sprague-Dawley rats were randomly allocated into two groups: control group, and hypoxia-treated group. Pups (n=30, respectively) in control and fetal hypoxia-treated group were examined specific binding of 125I-Tyr-nociceptin in cingulated gyrus, parietal cortex, hippocampus CA-1, CA-2, CA-3, and midbrain of postnatal day 2, 7, 14, 28, and 56 by the autoradiogram. The final amount of 125I-Tyr-nociceptin binding capacity was calculated densitometrically using Molecular AnalystTM version 1.4.1 (Bio- Rad, Hercules, CA, USA).
Result: At postnatal day 2 (P2), the rank order of the specific binding of 125I-Tyr-nociceptin was parietal cortex, cingulated gyrus, hippocampal CA-3, midbrain, hippocampal CA-1 and CA-2 area. In cingulated gyrus of the control rats, the specific binding of 125ITyr- nociceptin was noticed at P2, reached to peak at P7, thereafter decreased to lower level
than the value of P2. The maximal density was decreased and delayed in hypoxia-treated rats and the specific binding of 125I-Tyr-nociceptin was lower than that of the control group. At P2, the specific binding of 125I-Tyr-nociceptin was highest among the brain areas examined and showed peak value at P14. At P2, the specific binding of 125I-Tyr-nociceptin in
hypoxia-treated rats was lower than the control value. The latency to peak density of noci-ceptin receptor was delayed to P14. The specific binding of 125I-Tyr-nociceptin was lower in hypoxia-treated rats than in control group.
Conclusions: These results elucidate the localization of nociceptin receptors in discrete brain area of developmental stages of the rats and the alteration of its density by fetal hypoxic treatment.
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KEYWORD
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Brain, Hypoxia, Nociceptin receptor, Rat
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